Whole-Body Imaging of a Hypercholesterolemic Female Zebrafish by Using Synchrotron X-Ray Micro-CT
Visualizing whole-volume of the zebrafish tissues is limited by pigmentation and hard tissues. The 3D microstructures of a high-cholesterol zebrafish are visualized by synchrotron X-ray micro-computed tomography(SR-μCT). Various microstructure organs of the zebrafish are observed by high-contrast staining and embedding a protocol to improve the image contrast of soft tissues. The internal organs and cells of a hypercholesterolemic zebrafish are compared. The SR- μCT is powerful method to understand the pathogenesis of circulatory vascular diseases by detecting the modifications in the 3D morphological structures of the whole body of the
Zebrafish compared to others.